Effect of Allium cepa seeds ethanolic extract on experimental polycystic ovary syndrome (PCOS) apoptosis induced by estradiol-valerate

Abstract

Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting about 5- 10% of women in age of procreation. Apoptotic effects of Allium cepa seeds ethanol extract on experimental PCO induced by estradiol-valerat (PPA) in rats. Wistar female rat (n=60) were allocated into three groups, control (n=30); C1, an equal volume of (0.9% NaCl); C2, extract (0.3cc/rat/orally/daily); C3, Sesame oil(0.3cc/rat/orally/daily) and test groups (n=30), that subdivided into groups of 3, one group received extract supplement (0.3cc Sesame oil+ 0.3cc Allium Cepa /rat/orally/daily),second and third groups were induced PCO by single injection of estradiolvalerate (4mg/rat/IM),third group received extract supplement, for 60 consequence days. Animals were kept in standard conditions. In last day of study the blood samples and ovarian tissue of rats in whole groups were removed and prepared to biochemical and pathology analysis. Means of hyperaemia, number of cyst and granulosa apoptotic cells were significantly increased in PCO groups (p<0.05), and these were significantly decreased in group receiving extract in comparison to experimental PCO and control groups. Level of TAC in all extract groups were significantly increased as compared to control and PCO groups (p<0.05). Means of Large antral follicle was significantly decreased in PCO groups (p<0.05). Results revealed that administration of Allium Cepa ethanol extract significantly compensation blood antioxidants level in PCO- induced rats that led to modulating the apoptosis.