Consumption of 5-hydroxymethylfurfural-rich dried fruits is associated with reduction in urinary excretion of 8-hydroxy-2?-deoxyguanosine: a randomized clinical trial

Abstract

5-Hydroxymethylfurfural is widely presented in foods and produced through the degradation of hexoses and the Maillard reaction. This study aimed to investigate any association between 5-HMF content of consumed dried fruits and urinary excretion of 8-hydroxydeoxyguanosine (8-OHdG). The study consisted of 75 healthy volunteers. After an overnight fasting, two urine samples were collected before and 5آ h after consumption of date, apricot, currant, plum and fruit bread, 100آ g each. Twenty-five samples from frequently consumed types of dried fruits were analyzed for 5-HMF with HPLC. Urinary 5-hydroxymethyl-2-furoic acid (HMFA) and 8-OHdG were determined using HPLC and ELISA, respectively. The amount of 5-HMF in the tested samples varied from 314.8آ آ±آ 166.1 to 2496.3آ آ±آ 2301.2آ mg/kg. The lowest and highest levels of 8-OHdG were 10.1آ آ±آ 1.5آ آµg/g creatinine for plum and 24.7آ آ±آ 6.6آ آµg/g creatinine for date. At the two time points, mean HMFA levels varied from 630.6آ آ±آ 114.5 to 2116.8آ آ±آ 600.3 and 286.7آ آ±آ 24.8 to 582.0آ آ±آ 119.2 (آµg/g creatinine), respectively. There was a significant difference in HMFA amounts of the samples before and after consumption of apricot and plum (Pآ <آ 0.05). Overall, an inverse association was observed between 5-HMF contents of consumed dried fruits and changes of 8-OHdG (rآ =آ ?0.41, Pآ <آ 0.01). Also, there was a straight relationship between changes of HMFA and changes of 8-OHdG for all the fruits tested (rآ >آ 0.66, Pآ <آ 0.01). It is concluded that consumption of 5-HMF-rich dried fruits is associated with a significant reduction in urinary excretion of 8-OHdG. é 2015, Springer-Verlag Berlin Heidelberg.