Comparison of morphological and morphometrical characteristics in the glandular epithelium of mouse endometrium in preimplantation period after administration HMG-HCG, progesterone and sildenafil citrate

Abstract

Background: Synchronous development of embryo and endometrium is an essential prerequisite for successful implantation. Progesterone and some other drugs help to maintain synchronous development of preimplantation embryo through its action on maternal uterus. The aim of this study was to assessment the changes in morphology and morphometrical characteristics of endometrium after injections of progesterone and sildenafil citrate in superovulated mice. Methods: Forty adult female mice were divided into 4 groups as: control, gonadotropin, gonadotropin + progesterone and gonadotropin + sildenafil citrate. In all 3 experimental groups the mice received 7.5 I.U HMG and later HCG. Then every two female mice with one male mouse put in one cage for mating. In two lat groups 1 mg/mouse progesterone and 3 mg/kg sildenafil citrate administrated in 24, 48, 72 hours interval, after HMG injection. Ninety six hours after HMG injection, the mice in 4 groups were sacrificed and their uterine specimens were prepared for light microscopic studies. Findings: The mean height of glandular epithelium cells was 11.66 آ± 1.54 ?m in control group, 11.10 آ± 0.77 ?m in gonadotropin group, 11.06 آ± 1.30 ?m in gonadotropin + progesterone group and 11.37 آ± 1.22 ?m in gonadotropin + sildenafil citrate. Finally, the heights of the cells in all groups were not significantly deferent from each other (P < 0.05 for all of the comparisons). Conclusion: Ovarian induction followed by progesterone and sildenafil citrate injection would not modify the morphometrical indices of glandular epithelium of mouse endometrium.