Analyses of phytosterols and free radical scavengers in the bulbs of Ornithogalum cuspidatum Bertol [Analisis de fitosteroles y captadores de radicales libres en bulbos de Ornithogalum cuspidatum Bertol]

Abstract

The gas chromatography-mass spectrometry (GC-MS) analyses of the methanol (MeOH) extract of the bulbs of Ornithogalum cuspidatum led to the identification of thirteen phytosterols, namely cholesterol, 3,5-didehydro-stigmastan-6,22-diene, 4,4-dimethyl-5?-cholest-7-en-3-one, 4?-methyl-cholesterol, 5-cholestene-3?,7?-diol, campesterol, cholest-4-ene-3,6-dione, stigmast-4-en-3-one, stigmasta-3,5-dien-7-one, stigmasterol, ?5-ergostenol, ?-sitosterol, ?-sitosterol The free radical scavenging activity of the MeOH extract, and its solid-phase extraction fractions were assessed by the 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay, and the 40% MeOH-water fraction showed the highest degree of free radical scavenging property (RC50 8.87 أ— 10-2 mg/mL), compared to that of the positive controls Trolox® and quercetin 3.07 أ— 10-3 and 2.78 أ— 10-4 mg/mL, respectively. The 40% MeOH-water fraction also had the highest level of total phenolics content. é 2010 Bolet?n Latinoamericano y del Caribe de Plantas Medicinales y Arom?ticas.