Histopathological and ultra structural lesions study of kidney of alloxan induced diabetes mellitus in German Shepherd's dog

Abstract

Diabetes mellitus is one of the most common disease of endocrine glands in body that is diagnosed by malfunction in natural metabolism of carbohydrate, fat and protein. This disease involves most tissues of the body and the consequent deficiencies reduce their efficiency, cause infections and disease in body. Alloxan is a chemical which is used in creating experimental diabetes in animals. In this research, 9 German shepherd dogs were provided, 5 of which was considered as our experimental group and the remaining 4 was considered as our control group. The necessary examinations were conducted to guarantee their health and we approved the absence of diabetes with Intravenous Tolerance Test (IVGTT). We injected intravenously Alloxan mono hydrate with 100 mg kg(-1) in experimental group. After approving the presence of diabetes by IVGTT, we reported clinical signs. We scrutinized, macroscopic, microscopic and ultra structural deficiencies of kidney and then we stated the result.