Restriction fragment length polymorphism of bovine growth hormone gene intron 3 and its association with testis biometry traits in Iranian Holstein bull

Abstract

The association of bovine growth hormone gene polymorphism with testis biometry trait as average testis length (ATL), average testis width (ATW) and scrotum circumference (SC) in Iranian Holstein bulls. PCR-RFLP method with Msp-I restriction enzyme was used for genotyping. The frequency of the MspI(+)(C) and MspI(-)(D) alleles are 0.883 and 0.117 respectively. The genotype frequency for CC, CD and DD were 0.787, 0.191. The CD genotype was omitted of analysis of testis biometry trait. Mixed model analyses of considering genotype and environment as fixed effects and animal as a random effect suggested that sire was a significant source of variation (P < 0.001) in all traits. The CC genotype resulted in a significant increase in (p < 0.0001), PTSM (p < 0.0001). SC (p=0.3841). These results indicate that new molecular markers associated with sperm quality traits can be used in marker-assisted selection in bulls.