Effect of rosmarinic acid on estrogen, FSH and LH in female diabetic rats

Abstract

Rosmarinic acid from the plants quenched superoxide radicals from xanthine oxidase and inhibited cyclooxygenase I and II enzymes. Antioxidants have essential effect on diabetes. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat (n=40) were allocated into three groups, control group (n=10), rosmarinic acid (Ro) group that received 5 mg/rat (gavage) (n=10) and diabetic group that received 55 mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided into two groups of 10; STZ group and treatment group. Treatment group received 55 mg/kg (IP) STZ plus 5 mg/rat Ro daily for 4 weeks, respectively. However, the control group just received an equal volume of distilled water daily (gavage). Diabetes was induced by a single (IP) injection of streptozotocin (55 mg/kg). Animals were kept in standard condition. In 28 days after inducing, diabetic 5 cc of blood were collected for sex hormones, TAC and MDA levels from the whole groups analysis. Level of MDA significantly decreased in group that has received 5 mg/rat of Ro (P<0.05) in comparison to experimental groups and sex hormones, and TAC significantly increased in groups that received Ro (P<0.05). Since in our study, 5 mg/rat of Ro, have significantly preventive effect on diabetic disorder in female sex hormone, so it seems that using it can be effective for treatment in diabetic Rat