Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe

Abstract

A spectrofluorimetric method is proposed for the determination of human serum albumin (HSA) and bovine serum albumin (BSA) using terbium-danofloxacin (Tb3+-Dano) as a fluorescent probe. These proteins remarkably enhance the fluorescence intensity of the Tb3+-Dano complex at 545 nm, and the enhanced fluorescence intensity of Tb3+-Dano is proportional to the concentration of proteins (HSA and BSA). Optimum conditions for the determination of HSA were investigated and found that the maximum response was observed at: pH = 7.8, [Tb3+] = 8.5 x 10(-5) mol L-1, [Dano] = 1.5 x 10(-4) mol L-1. The calibration graphs for standard solutions of BSA, HSA, and plasma samples of HSA were linear in the range of 0.2 x 10(-6) - 1.3 x 10(-6) mol L-1, 0.2 x 10(-6) - 1.4 x 10(-6) mol L-1, and 0.2 x 10(-6) - 1 x 10(-6) mol L-1, respectively. The detection limits (S/N = 3) for BSA, HSA, and plasma sample of HSA were 8.7 x 10(-8) mol L-1, 6.2 x 10(-8) mol L-1, and 8.1 x 10(-8) mol L-1, respectively. The applicability of the method was checked using a number of real biological plasma samples and was compared with the UV spectrometric reference method. The results was showed that the method could be regarded as a simple, practical, and sensitive alternative method for determination of albumin in biological samples.