THE PREVALENCE OF HELICOBACTER PYLORI CAGA AND ICEA GENOTYPES AND POSSIBLE CLINICAL OUTCOMES

Abstract

Objective: There is continuing interest in identifying Helicobacter pylori virulence factors that might predict the risk for symptomatic clinical outcomes. It has been proposed that iceA and cagA genes are such markers and can identify patients with peptic ulcers and gastric cancer. Methods: To determine the prevalence of specific genotypes of H. pylori, clinical isolate of H.pylori obtained from 102 patients through endoscopic biopsies was cultured. The cagA alleles, iceA genotypes were determined by PCR. Results: Distribution of cagA and clinical outcome was shown that the frequency of cagA-positive isolates in PUD, NUD and GC patients was 81.25% and 65.5% and 100%, respectively. Also the iceA1 allele was identified in 2 (100%) GC patients but iceA2 allele was not detected in these patients. Overall cagA and iceA1 alleles were detected in GC patients. Conclusions: The cagA gene and iceA1 genotype was found to predominate in gastric adenocarcinoma patients, and also iceA2 genotype was also associated with PUD.