Analysis of human B cell response to recombinant Leishmania LPG3

Abstract

Objective: To evaluate the capability of recombinant Leishmania LPG3 and its fragments in the activation of B cells. Methods: In the present study, human B cells were purified from peripheral blood of 10 adult healthy subjects using magnetic-activated cell sorting technique. Subsequently, purified B cells were treated with recombinant LPG3, and its AT-terminal and C-terminal fragments at different concentrations (2, 10 and 20 mu g/mL). B cell activation was assessed through expression of CD69 molecule by flowcytometry and secretion of IL-6, TNF-alpha and IL-10 cytokines via enzyme-linked immunosorbent assay following treatment with recombinant antigens. Results: Our results showed that while the recombinant LPG-3 could significantly increase the production of IL-6 and TNF-alpha (P<0.05) in B cells, it had no effect on the secretion of IL-10 by B cells. Conclusions: Our study indicated that recombinant LPG-3 and especially its N terminal fragment could stimulate B cell response as an important immune response component against leishrnaniasis. Thus, it seems that it can be considered as an effective adjuvant in vaccine developments against leishmaniasis.