Fecal Carriage of ES beta L types TEM, SHV, CTX Producing Genera Proteus, Morganella, Providencia in Patients of Iran

Abstract

Diseases like urinary tract infection, wound infections, bacteremia and other infections are mainly caused by the members of the genus Proteus, Morganella and Providencia which are mainly either found freely in the environment or in the gastrointestinal tract of humans. We studied Fecal carriage of ESPE producing species in carrier patients.Stool samples obtained from outpatients and inpatients not suffering from diarrhea and were cultured in CTX-MC-Conkey agar. Lactose negative and cefotaxime resistant bacteria were identified by biochemical tests and ES beta L-producing isolates were detected using Combined Test. TEM, SHV and CTX genes were investigated by PCR.Total 15 (7.35%) isolates of 204 stool samples were identified as ESBL producing Proteus spp. (n=4,1.96%), Morganella spp. (n=5, 2.45%) and Providencia spp. (n=6, 2.94%). Further, amongst or of the 15 ES beta L producing strains, blaTEM was the commonest genotype (86.66%), followed by blaSHV (26.66%) and blaCTX-M (20%). All isolates were resistant to ampicillin, and cefotaxime whereas all Providencia and Morganella spp. were found to resist ceftazidime. Although the number of ES beta L-producing Proteus, Morganella and Providencia isolates from fecal carriers were low, but still, they can be considered as a reservoir of TEM, SHVand CTX genes and capable to transfer these resistant bacteria to hospitals.