EVALUATION OF AMINOGLYCOSIDES RESISTANCE GENES AMONG BETA LACTAMASEPRODUCING ESCHERICHIA COLI

Abstract

The aim of this study was to determine the occurrence of Aminoglycoside resistance in relation to extended-spectrum B-lactamase(ESBL)producing Escherichia coli in Tabriz. Escherichia coli (E. coli) is a member of human and animals gut flora. It is also one of the major causes of nosocomial and other infections. Aminoglycosides are one of the antibiotics groups which are used for treatment of E. coli infections and yet resistance to aminoglycosides has increased in the recent years. ESBL-producing E. coli are specifically concerning because they confer resistance to a large number of antibiotics including third generation cephalosporin. The present study investigated the prevalence of genes encoding aminoglycoside-modifying enzymes in ESBL producing E. coli strains isolated from intestinal or extra-intestinal infections. Combined disk procedure was carried out for detection of beta lactamase production using ceftazidime and ceftazidime/clavunic acid. The frequency of aminoglycoside modifying enzymes encoding genes aac (3)-IIa, ant(2')-Ia, aph(3')-IIa, aph(3')-Ia, and aac(3)-IV was analyzed by the PCR method. The results of this study indicated that the genes encoding aminoglycoside-modifying enzymes are prevalent in E. coli isolates in the study region, which highlighted the necessity of considering preventive measures to control dissemination of these resistance genes.