• English
    • Persian
    • English
    • Persian
  • English 
    • English
    • Persian
    • English
    • Persian
  • Login
View Item 
  •   KR-TBZMED Home
  • TBZMED Published Academics Works
  • Published Articles
  • View Item
  •   KR-TBZMED Home
  • TBZMED Published Academics Works
  • Published Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Vitamin D-Loaded Nanostructured Lipid Carrier (NLC): A New Strategy for Enhancing Efficacy of Doxorubicin in Breast Cancer Treatment

Thumbnail
Date
2017
Author
Sabzichi, M
Mohammadian, J
Mohammadi, M
Jahanfar, F
Pour, AAM
Hamishehkar, H
Ostad-Rahimi, A
Metadata
Show full item record
Abstract
The dangerous dose-dependent side effects of anticancer agents triggered the finding of new approaches for elevated chemotherapy efficacy. This study investigated the potential application of nanostructured lipid careers (NLCs) in increasing vitamin D-3 (VitD) effectiveness in breast cancer cell (MCF-7) in concurrent administration with doxorubicin (Dox). VitD-loaded NLCs were characterized by particle size, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscope. Cytotoxicity and molecular effects of formulation were evaluated by MTT, DAPI staining, flow cytometry, and real-time quantitative PCR assays. The formulation revealed mean particle size of 87 +/- 5nm with a polydispersity index of 0.24 confirmed by SEM images. The IC50 values for VitD and Dox were 1.3 +/- 0.04 and 0.65 +/- 0.05 mu M, respectively. VitD-loaded NLCs decreased the percentage of cell proliferation from 49 +/- 7.2% to 37 +/- 5.1% (P < 0.05). Cotreatment of the cells with VitD-loaded NLCs and Dox caused over a twofold increase in the percentage of apoptosis (P < 0.05). Gene expression profile demonstrated a significant decrease in antiapoptotic factor survivin along with increase in proapoptotic factor Bax mRNA levels. Overall, our results introduced the NLC technology as a novel strategy to elevate the efficacy of chemotherapeutics in breast cancer.
URI
http://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/46116
Collections
  • Published Articles

Knowledge repository of Tabriz University of Medical Sciences using DSpace software copyright © 2018  HTMLMAP
Contact Us | Send Feedback
Theme by 
Atmire NV
 

 

Browse

All of KR-TBZMEDCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

Knowledge repository of Tabriz University of Medical Sciences using DSpace software copyright © 2018  HTMLMAP
Contact Us | Send Feedback
Theme by 
Atmire NV