Current advancement in immunosensing of p53 tumor suppressor protein based on nanomaterials: Analytical approach

Abstract

The utility of detection of p53 in tomorrow's personalized health care field will mean early and accurate diagnosis of many types of cancers. This review is meant to provide an overview of the various types of immunosensors and immunoassay have been developed for determination of p53, along with significant advances over the last several years in related technologies. In addition, this review described: Most frequently applied principles in immunoassay/immunosensing of p53. The aspects of fabrication in the perspective of immunoassay/immunosensing applications. The potential of various electrochemical, electrochemiluminescence immunosensors for the determination of p53 within sub-nanomolar range and the circumvention of the most serious problem in immunosensing will be discussed. Some of multiplex electrochemical immunosensors have been discussed with and without labels. We also summarize the latest developments in the applications of immunoassay/immunosensing methods for detection of p53 in real samples. The development trends of electrochemical and electrochemiluminescence based immunosensors are also introduced, including newly developed integrated immunosensors, and the application of nanotechnology and microfluidic technology. (C) 2017 Elsevier B.V. All rights reserved.