Role of HDM2 Gene in Radio-Sensitivity of Esophageal Cancer Cell Lines to Irradiation

Abstract

Background: Radio therapy plays an important role in controlling tumor growth in esophageal cancer patients. Objectives: Our study provided comprehensive information about radio-sensitivity of Hdm2 gene in esophageal cancer cells in cancer cells. Methods: The expression of Hdm2 gene in radiosensitive and radio-resistant esophageal squamous cell carcinoma TE1, TE8 and TE11 cell lines is studied. RNA extracted from each esophageal cancer cell, then Quantitative reverse transcription PCR (RT-qPCR) assay were used for measuring HDM2 gene expression. Finally, radiosensitivity was determined for every three esophageal cancer cell lines. Results: It was found that Hdm2 gene significantly expressed in TE1, TE8 and TE11 cell lines by qRT-PCR analysis. Clonogenic assay showed that TE-11 was very sensitive to radiation relative to other cell lines. Conclusions: Hdm2 protein binding to the P53 protein is a key regulator of cell cycle control and genome protection Cell cycle control, programmed cell death and response cells to radition play important roles.