Elevated Serum 8-Hydroxy-2 '-Deoxyguanosine, Nitrite, and Nitrate in Patients with Stage I Multiple Myeloma

Abstract

Background: Reactive oxygen species are highly active intermediates, which lead to cellular damage during imbalance between oxidative stress/antioxidant towards oxidative stress. Free radicals have been proposed to have a crucial role in development and progression of human cancer. Objectives: The main aim of this study was to assess the changes in 8-hydroxy-deoxyguanosine (8-OHdG), nitrite (NO2-), and nitrate (NO3-) levels in patients with Stage I multiple myeloma (MM). Methods: The serum samples were obtained from 34 patients with stage IMM(22 males and 12 females at stage I) who were matched with healthy control group. Total serum of NO2- and NO3- levels, in addition to serum 8-OHdG levels, were assayed by using the commercial assay kit. Results: Compared with the healthy case group, the levels of serum NO2- and NO3-(2.54 +/- 0.49 vs. 1.75 +/- 0.52 +/- mol/L, P < 0.001 and 6.37 +/- 0.94 vs. 2.63 +/- 1.06 mu mol/L, respectively, P < 0.001) were significantly increased in patients with MM. Higher levels of 8-OHdG in MM patients (157.08 +/- 13.61 pg/mL) were observed and compared to the control group (61.48 +/- 9.93 pg/mL) and indicated that 8-OHdG levels in patients with cancer were significantly increased than those of the control group (P < 0.001). Conclusions: Significantly increase in oxidative DNA damage and end-products of nitric oxide (NO) metabolism levels in patients with stage I MM suggest the possible involvement of oxidative stress in MM disease. These alterations can be associated with the imbalance in oxidant-antioxidant status in these patients and may contribute to carcinogenesis of MM by oxidative DNA damage.