The effect of estradiol benzoate and FSH on hormonal levels and stereology structure of testis in Ghezel lambs treated with Tamoxifen citrate
چکیده
This study was conducted to evaluate the effect of estradiol benzoate (EB) and FSH on hormonal levels and stereology structure of testis in Ghezel lambs treated with Tamoxifen citrate (TC). Sixteen lambs (105-110 days old) were randomly allocated in two groups. The control group (4 lambs) received distilled water (5 ml/day) by oral gavage for 30 days). The treatment group (T 12 lambs) received first Tamoxifen citrate (660 mu g/kg by oral gavage for 30 days), and was then allocated into three groups: T-T that received TC (660 mu g/kg by oral gavage), T-FSH that received FSH (3 mg/ kg, by IM), and T-EB that received EB (3 mg/kg, by IM) for 10 days. The stereological evaluation of the lamb testes showed that the groups T-T, TCFSH and TC-EB showed extensive seminiferous tubular atrophy compared with control group. The administration of TC with EB significantly (P<0.01) improved the volume of germinal epithelium compered to TT group but co-administration of FSH did not induce significant differences in this parameter, in comparison with T-T group (P<0.01). The administration of TC increased the intertubular space, reduced testicular weight, the height of the germinal epithelium, spermatogenic and sertoli-leydig cells number (P<0.01). There was no significant variation on the volume of testis (length, height and width), seminiferous tubule, lumen, capsule and interstitium (P> 0.01). Serum testosterone and FSH concentrations were significantly reduced (P<0.01) when lambs were treated with TC 20 mg/kg daily for 30 days. These finding demonstrated that FSH and EB could be useful in improving testicular structure and function of testis following TC treatment.