Evaluation mannose-binding lectin gene and promoter polymorphism in renal transplant recipients.

Abstract

The aim of present study was to determine the distribution of the alleles of mannose-binding lectin gene and promoter variants in renal transplant recipients and seek correlation between these variants and diseases that cause renal dysfunctions.One hundred and thirteen renal recipients' samples were compared with 120 normal controls from Azarbaijan population of Iran. Blood samples were obtained from renal transplant recipients who received a kidney between March 2004 and July 2005. Mannose-binding lectin genotypes were investigated by polymerase chain reaction and restriction fragment length polymorphism.Allelic and genotypic frequency of the polymorphism at position- 550, -221, +4 and at codon 52, 54 and 57 did not show statistical differences between recipients and controls (P > 0.05) but significant frequency of allele B (codon 54) (P = 0.02) and Lx haplotype (P = 0.002) of promoter was observed in patients with Lupus Erythematosus and infection source of renal dysfunctions.Our findings provide evidence that presence of different alleles and haplotypes that cause low concentration of mannose-binding lectin in serum is a risk factor for severity of systemic Lupus Erythematosus and susceptibility to renal infections that cause renal dysfunction.