Indirect coculture of stem cells with fetal chondrons using PCL electrospun nanofiber scaffolds.
تاریخ
2017نویسنده
Nikpou, P
Soleimani Rad, J
Mohammad Nejad, D
Samadi, N
Roshangar, L
Navali, AM
Shafaei, H
Nozad Charoudeh, H
Danandeh Oskoei, N
Soleimani Rad, S
Metadata
نمایش پرونده کامل آیتمچکیده
In vitro coculture system provides a powerful tool for tissue engineering. In this study, we evaluated the gene expressions of human adipose-derived stem cells (ASCs) on polycaprolactone (PCL) scaffold in coculture model with fetal chondrons. Electrospun PCL scaffolds (900?nm fiber diameter) were created and human infrapatellar fat pad-adipose-derived stem cells (IPFP-ASCs) were seeded on these scaffolds. Scanning electron microscopy (SEM) showed attachment of human IPFP-ASCs to scaffold. IPFP-ASCs on scaffolds were cocultured with fetal chondrons in transwell. Gene expressions were investigated using real-time polymerase chain reaction (real-time PCR). In comparison with control group, the expression level of collagen type 2 and aggrecan were significantly decreased but Indian Hedgehog(IHH) significantly increased (P?<?0.05).These findings may interpreted that IPFP-ASCs seeded on PCL scaffold, in cocultures with fetal chondrons are tending toward osteogenesis rather than chondrogenesis.