Design and Development of Modified mRNA Encoding Core Antigen of Hepatitis C Virus: a Possible Application in Vaccine Production

Abstract

Hepatitis C virus (HCV) is a "ژblood-borne pathogen, resulting in liver cirrhosis and liver cancer. Despite of many efforts in development of treatments for HCV, no vaccine has been licensed yet. The purpose of this study was "ژto design and prepare a specific mRNA, without 5' cap and poly (A) tail transcribed in vitro capable of coding core protein and also to determine its functionality.Candidate mRNA was prepared by in vitro transcription of the designed construct consisting of "ژ"ژ5? and 3? untranslated regions of heat shock proteins 70 (hsp70) mRNA, T7 promoter, internal ribosome entry site (IRES) sequences of eIF4G related to human dendritic cells (DCs), and the "ژCore gene of HCV. To design the modified mRNA, the "ژ"ژ5' cap and poly (A) tail structure was not considered. DCs were transfected by in vitro-transcribed messenger RNA (IVT-mRNA) and the expressions of green fluorescent protein (GFP), and Core genes were determined by microscopic examination and Western blotting assay.Cell transfection results showed that despite the absence of "ژ"ژ5' cap and poly (A) tail, the structure of the mRNA "ژwas stable. Moreover, the successful expressions of GFP and Core genes were achieved.Our findings indicated the effectiveness of a designed IVT-mRNA harboring the Core gene of HCV in transfecting and expressing the antigens in DCs. Considering the simple and efficient protocol for the preparation of this IVT-mRNA and its effectiveness in expressing the gene that it carries, this IVT-mRNA could be suitable for development of an RNA vaccine against HCV.