سنتز و ارزیابی نانو پارتیکل های حساس به دمای حامل سیپروفلوکساسین برای دارورسانی طولانی رهش چشمی

Abstract

Introduction: Bacterial infections are a major source of keratitis. Ciprofloxacin has been shown to be generally effective in treating bacterial keratitis. An ideal ocular drug delivery system should possess good loading capacity, ability to reside in the precorneal area for a substantial period, and capacity to release the drugs in a controlled manner. Colloidal nanocarriers have been successfully employed to enhance intraocular drug penetration and controlled release. Aim: The purpose of this study was to prepare and characterize an ocular effective prolonged-release thermosensitive in-situ forming hydrogel formulation containing ciprofloxacin for the treatment of keratitis. Methods: Crosslinked poly (NIPAAm-MAA-VP) copolymer was synthesized and used for preparation of ciprofloxacin-loaded hydrogel nanoparticles. Antimicrobial effect of the drug nanoformulation was studied in comparison to marketed solutions of ciprofloxacin using Pseudomonas aueroginosa using MIC determination. For in-vivo animal studies rabbits were selected as appropriate animal model of interest to induce keratitis. Results: The keratitis surface area over the course of disease varied according to the treatment types. The keratitis in starting control rabbits developed total wound extension over the study period. Keratitis wound area increased from a mean of ۳.۸ mm۲ at the second day up to ۵.۷ mm۲ at day ۶. After extension of the keratitis wound up to the sixth day of infection the reduction of wound surface area from day ۶ to day ۱۴ was compared for the three trial modalities.Conclusion: Nanoformulation of ciprofloxacin had a higher efficacy in treating bacterial keratitis when compared to control treatment in severe forms of keratitis in an animal model