The effect of anti-bacterial CO2 Laser and 0.2 percent chlorhexidine solution on staphylococcus aureus and Pseudomonas aerginosa within 24 and 48 hours of laboratory environment

Abstract

The bacterium causes different diseases in the oral cavity including angular cheilitis, bacterial sialoadenitis in salivary glands, joint septic arthritis (Tmj). In addition, the accumulation of bacteria on the surface of the implant develops the inflammation of mucous. The aim of this study was to compare the effects of CO2 laser with 2.0 percent chlorhexidine in laboratory environment for eradicating Staphylococcus aureus and Pseudomonas aeruginosa Methods and materials: The effect of CO2 Laser was evaluated 5, 10, and 15 seconds after exposure to the standard suspension of bacterium at 24 and 48 hours intervals ,bacteria were cultured until the logarithmic phase and CO2 laser radiation with the wavelengths of 6 and 10 ?m and energy density of 12.5 J/cm2 and 0.5 w were radiated at a distance of 17mm. Chlorhexidine 0.2? also was assessed at 5, 10, 15, and 60 seconds on the pathogens. Data was analyzed by descriptive statistics (mean SD) and repeated measures ANOVA (RMANOVA) using SPSS.17. The P-value of less than 0.05 was considered statistically significant. Results: Co2 laser radiation was effective at all less than 15 seconds radiations on reducing both types of bacteria while the average numbers of Staphylococcus aureus which were affected by 15 seconds of laser radiation and 60 seconds of chlorhexidine showed statistically significant difference (P-value= 0.014). The numbers of Pseudomonas aeruginosa bacteria in the group which was exposed by laser for 15 seconds, and the group which was treated by chlorhexidine were zero. Whereas the average number of bacteria of laser treated group was lower than 0.2? chlorhexidine at both groups of bacteria. Laser radiation removed 100? of bacteria after 15 seconds in both groups of the bacteria. Conclusions: The antimicrobial effect of CO2 laser and chlorhexidine0.2? was obvius on the Staphylococcus aureus and Pseudomonas aeruginosa at laboratory conditions Findings of the present study indicates that laser irradiation for 15 seconds had equal results with chlorhexidine and more effective than 60 seconds treatment with chlorhexidine.