The effect of adding containing Silica fume on cytotoxity of white mineral trioxide aggregate

Abstract

Mineral Trioxide Aggregate (MTA), as a highly biocompatible biomaterial has many applications in dental treatments. Studies have revealed similar structure of MTA and portland cement. Recent studies also have shown improved physical properties of portland cement by addition of containing silica fume (CSF) . Therefore, the addition of CSF may theorethically improve the properties of MTA. The aim of the present study was to evaluate the cytotoxicity of MTA mixed with CSF on L929 mouse fibroblast with tetrazolium bromide reduction assay (MTT assay). Materials & Methods: White MTA (ProRoot, Dentsply) was mixed with distilled water according to the manufacture instructions. Mixtures of White MTA with 10,15 and 20% CSF by weight were prepared and mixed with distilled water . in 1:3 proportion. 24 hour-set specimens were eluted with culture medium according to ISO 10993:5 standard. Distilled water and culture medium served as positive and negative control respectively. Cytotoxicity of mixtures was compared with MTT assay on L929 mouse fibroblast cell line after 24,48 and 72 hours. Differnces in cytotoxicity were assesed by one way ANOVA. Results: Means and standard deviations of vital cell counts cultured in MTA , MTA mixed with 5% CSF, MTA mixed with 10% CSF, and MTA mixed with 15% CSF were 98%6, 97%6, 94%4 & 98%4 respectively . One Way ANOVA did not reveal any statistically significant differences between the groups (P>0.05). Conclusion: Within the limitations of this in vitro study, it may be concluded that addition of CSF to MTA does not influence its cytotoxicity. It is suggested that the influence of CSF on other proporties of MTA be evaluated in future studies.