Evaluation of the film thickness and antibacterial property of Mineral trioxide aggregate mixed with propylene glycol as a root canal sealerat different time intervals

Abstract

The aims of this study were to compare the film thickness andantibacterial property of MTA-PG as a sealer in comparison with MTA-fillapex and AH26 sealer.Materials and Methods: The antibacterial property of the sealers was evaluated using direct contact test(DCT). Eight micro-tube were used for testing antibacterial property in fresh state. One-fourth of the tubes were filled with freshly mixed sealers. After 20minutes,10µl ofE.faecalis (ATCC 29212) bacterial suspension was added to the tubes. Following one-hour incubation, sealers were floated in 500µlBHI broth culture medium. After 10 minutes and one-hour,10µl of the tube BHI was transferred to BHI agar culture plates and incubated. After 24 hours mean colony forming units(CFU) was counted. The antibacterial property in set state was assessed using the above-mentioned process except that,the bacterial suspension was added to the tubes after 72hours.Film thickness of sealers was measured according to ISO 6876/2012.The data were statistically analyzed usingDescriptive statistics (mean ± SD)and repeated measures of ANOVA..Results: CFU means in AH26 was significantly more than other groups (p<0.0001), but there was no significant difference between the CFU means of MTA-PG and MTA-fillapex, (p>0.05). Mean of CFUs in set AH26 after one hour exposure was significantly more than 10 min exposure(p=0.006)..Conclusion: MTA fillapex and MTA-PG showed complete anti-bacterial effect. The anti-bacterial effect of AH26 sealer in the fresh state was remarkably more than 72-h set state. The film thickness of MTA-PG and MTA fillapex was significantly less than AH26