Evaluation of Photo Activated Disinfection effect on candida albicans growth by different concentration of toluidine blue in vitro

Abstract

Candida albicans is one of the components of the normal flora of the mouth and oral mucosa and the most common its opportunistic infections. The widespread use of topical and systemic antifungal agents as conventional treatment for oral candidosis has resulted in the development of resistance in C. albicans. One of promising modality for overcome to this problem is photodynamic therapy (PDT).Moreover, Finding the lowest appropriate conncentration of toluidine blue that has the ability to influence the PAD is valuable. This invitro study, investigated the effects of photodynamic therapy with toluidine blue on Candida albicans. Materials & Methods: In this experimental study, the 8 test tubes with suspensions of C.albicans standard strains were selected. All tubes had toluidine blue but with various concentrations. PAD had done on number 5 to 8 test tubes, after that C.albicans suspensions of 8 test tubes were cultured on saburaual dextrose agar. After incubation, the number of colony in every plate was Investigated . In the final step ,results of counting the number of colonies in suspensions of 8 test tubes were compared each other. Data was analyzed, using TWO WAY ANOVA test and P<0.05 was regarded statistically meaningful. Results: PAD with an increase in TB concentration from 0 to 6.35 mg/l reduce the Candida Albicans population significantly But reduction was not statistically significant in 12.7 mg/l concentration. Moreover, accordig to results of this study, the least effective concentration of TB without PAD is 6.35 mg/L. Conclusions: Our invitro study findings, show that PAD in presence of toluidine blue with 6.35 mg/l concentration more effectively can reduce the number of candida albicans