Determination of Mast cell and microvessel counts in dentigerous cyst, odontogenic keratocyst and periapical cyst

Abstract

Cysts are the most common destructive lesions of the jaw bones. Odontogenic cysts represent the most common form of jaw cysts. Periapical cysts, dentigerous cysts, and odontogenic keratocysts collectively represent up to 95% of odontogenic cysts. The precise roles of mast cells and angiogenesis in these lesions are not defined. Purpose of study: The purpose of this study was to investigate mast cell and micro vesssel densities in odontogenic cysts. Also we evaluated there relation between mast cells and microvessels in these lesion to clarify the role of these cells in angiogenesis and cyst growth. Methods: In this cross-sectional study, 60 samples, including radicular cyst (RC), dentigerous cyst (DC), odontogenic keratocyst (OKC) categories (20 for each), were selected .Twenty dental follicle of impacted third molars were considered as the control group. Immunohistochemical staining was performed. The number of mast cells and microvessels were counted in 5 high power fields beneath epithelium and 5 high power fields in depth of connective tissue ,containing maximum counts for each sample . A total mean (the mean of beneath epithelium and depth) was counted to know mast cell and microvessel densities in each sample. Results: Mast cell tryptase and CD31 expression means were significantly increased in radicular cyst compared to other cysts (P value <0.05). A significant correlation was not obtained between mast cell tryptase and CD31 expression in odontogenic cyst (P value >0.05). Conclusion: Inspite of presence of mast cells in odontogenic cysts, these cells do not play an active role in the growth of these lesions, so we can not use mast cell inhibitors for treatment purposes.