Comparison of salivary bacterial flora of patients with hiatal hernia versus healthy people

Abstract

Effect of several illnesses on salivary microbial flora has been surveyed and it has been shown that there is a relation between variation of oral flora and some illnesses in humanEs body. Hiatal hernia is a condition in which parts of the abdominal contents, mainly the GEJ and the stomach, are proximally displaced above the diaphragm through the esophageal hiatus into the mediastinum. Each type of hiatal hernia presents with different symptoms. H.H. is closely related to GERD. The most common symptom of GERD is heart burn which is described as sub sternal burning that moves from the epigastria to the neck. This patients are often asymptomatic and their discomfort is often when stomach acid or air or bile reflux occur. Changes in volume and composition of saliva in hiatal hernia patients seem to cause alternations in oral resident bacteria. No surveys have been done about oral microbial flora changes in the hiatal hernia patients so far, so in this study with checking the oral flora of the hiatal hernia patients, the comparison of salivary microbial flora of these patients and control group have been done. In this study 50 H.H. patients and 50 control groups in the range of 20-75 years old that have done endoscopy in Imam Reza hospital during 1393 and diagnosis shows the H.H. bigger than 1 Cm and no H.H. have been studied. 1 cc salivary sample of two groups before getting any medication and endoscopy get in sterile tubes and samples were transported to laboratory of Tabriz medical faculty immediately. We get 0.05 ml of saliva and transferred it to special mediums of viridans Streptococcus, Enterococcus and Lactobacilli. The mediums were incubated in 37 c for 7 days and then the colonies were checked and enumerated. Salivary smear was studied about Gram classification with Gram stain. Results show that Lactobacilli Fermentis, acidophilus, S. Mutans, Sanguis and Enterococcus were more in patients group and Lactobacilli Casei, S. Mitis and Salivarius were more in control group. but none of the differences were statically significant.