Comparison of bacterial leakage of mineral trioxide aggregate with and without silver nano-particles in root end cavities prepared with ultrasonic method

Abstract

Most of endodontic failures are associated with incomplete cleaning of root canal system and consequently penetration of bacteria and other antigens into periradicular tissues. Thus, besides the sealing ability and biocompatibility, root-end filling materials should have antimicrobial activity to halt microbial growth. Based on the previous studies which depicted desirable properties of the MTA and silver nano-particles mixture ( solubility and cytotoxicity of MTA with 1% silver nano-particles was similar to MTA itself while improved its antimicrobial activity.) this study was aimed to evaluate the sealing efficacy of mineral trioxide aggregate with and without silver nano-particles in root end cavities prepared by ultrasonic. Materials and Methods: This experimental study was carried out with seventy root-end cavities of human extracted teeth. After apical resection, the root-end cavities were prepared by ultrasonic retrotips. Teeth were randomly divided into 4 groups containing two experimental groups (n=30) and two negative and positive controls (n=5). In group 1 and 2, root-end cavities were filled with MTA and MTA with silver nano-particles (by 1% weight), respectively. Leakage assessment was carried out by bacterial leakage apparatus with Enterococcus faecalis species. Leakage comparison between experimental groups was done using U-Mann Whitney analysis by Spss 16 statistical software at significancy level of 0.05. Results: The mean bacterial leakage day for MTA and MTA-SN was 30.06 (28.67) and 9.66 (14.65), respectively. U Mann Whitney analysis indicate that there is a significant difference in bacterial leakage between two experimental groups (P=0.002). and MTANS leaked faster than MTA group. Conclusions: Based on the findings of this in-vitro bacterial leakage study, adding silver nano-particles to MTA decreased its sealing ability