Comparison of antibacterial efficacy of polymer containing silver nanoparticles with chlorhexidine 2% and sodium hypochlorite 2.5% solutions on E.faecalis in infected root canals

Abstract

Cleaning and shaping of root canal as well as using appropriate irrigants and intra-canal medications would reduce the microorganisms and increase the success rate of root canal therapy. Therefore, this study was aimed to evaluate the antibacterial properties of PLGA polymer containing silver nanoparticles (30 ppm) in infected roots with Enterococcus faecalis and compare this effect with sodium hypochlorite 2.5% and chlorhexidine 2%. Materials and methods: Antibacterial effect of silver nano-particle containing polymer against Enterococcus faecalis were evaluated using agar diffusion method and compared with sodium hypochlorite 2.5% and chlorhexidine 2%. After colony counting, the comparison of CFU among experimental groups was performed using Kruskal-Wallis analysis. Bonferroni post-hoc was utilized for pair-wise comparisons. P<0.05 was considered statistically significant in this study. Results: Kruskal-Wallis analysis revealed that there is a significant different between the CFU of tested antimicrobial agents. Pair-wise comparison using Bonferroni post-hoc indicated significant difference among all pair-wise comparisons except for normal saline with polymer and hypochlorite with chlorhexidine (P=0.000). Conclusions: silver nano-particles bear antibacterial effects against E. faecalis and antibacterial effect of Ag-polymer hydrogel was noticebility greater that the silver nano-particles aquas solution with 5% DMSO.