Biocompatibility evaluation of white Mineral Trioxide Aggregated with Titanium dioxide nanoparticles on gingival fibroblasts by MTT assay

Abstract

Mineral Trioxide Aggregate (MTA) is used for filling of root end cavities, direct pulp capping, repairing furcation perforations and apexification .Therefore, improving its physical properties and their stability is of great importance to ameliorate endodontic treatments. There are some studies about adding TiO2 nanoparticles to different materials since these particles have significant mechanical properties that can help improve physical properties of these materials. The purpose of this study was to find adequate and nontoxic dose of MTA with TiO2 nanoparticles, considering on gingival fibroblasts. Materials and methods: For studying tissue compatibility, this material (MTA with TiO2 nanoparticles ) was added to gingival fibroblast cell culture perimeter, in different density. Cellular changes after 24 and 48 hours, were studied by READER ELISA due to MTT assay protocol. Obtained data were presented as frequency and one,two way ANOVA. P value< 0.05 is considered significant. Results: The data of this study was evaluated by one,two way ANOVA test and MTA with TiO2 nanoparticles, did not have toxic effect on gingival fibroblast cells after 24 and 48 hours. Conclusion: Based on the findings of this in vitro study, addition of TiO2 nanoparticles to white MTA had no cytotoxic effects on fibroblast cells by MTT Assay.