Biocompability of freshly mixed mineral trioxide aggregate with and without adding nano silver particles in rat connective tissue

Abstract

Biocompatibilty and antimicrobial properties of materials used in endodontics are of utmost importance. Since extensive applications of Mineral Trioxide Aggregate (WMTA) in endodontics as well as antimicrobial features of silver nanoparticles, this study aimed to evaluate the subcutaneous inflammatory reaction of rat to WMTA with and without silver nanoparticles. Materials & Methods: In this study 75 male rat were divided into five groups (n=15) and the polyethylene tubes containing experimental materials (WMTA with silver nanoparticles (WMTANS), WMTA, and empty tubes (control)) were implanted in subcutaneous tissue. After 7, 15, 30, 60, and 90 days after tubes placement, the animals were sacrificed and the inflammatory reaction was graded. The collected data was analysed using Kruskal-Wallis and Mann Whitny tests. The P<0.05 was assumed statistically significant in this study. Results: The comparison of cumulative inflammatory reaction in all study intervals revealed that the median of inflammatory reaction of WMTANS, WMTA, and control were 3, 2, and 2, respectively. The analysis of Mann Whitney indicated that there was not any significant difference in inflammatory grades of WMTNS and WMTA during the study intervals (P>0.05). Conclusions: Based on the findings of this study, the inflammatory reaction of WMTANS and WMTA was the same.