A comparative evaluation of antimicrobial efficacy of four endodontic sealers against Enterococous faecalis

Abstract

Antimicrobial properties of endodontic sealers would influence the success rate of root canal therapy. Therefore the purpose of this study was to compare the antimicrobial effect of MTA Fillapex,Epiphany,Dorifill and Apexit plus on Enterococcus faecalis by Agar Diffusion Test (ADT). Methods and Materials:Ten plates were cultured with E. faecalis suspension(1.5 10 8CFU/ml) on Muller Hinton agar. five pits were prepared on medium surface by metal punch and the freshly mixed sealers were applied to four of them. The fifth pit was maintained empty as positive control. After incubation , diameter of the bacterial growth-free area around sealers was measured at 3, 5, and 7-day intervals. Results: Statistical analysis revealed that there was a significant difference in growth-inhibition area grade among all experimental sealers grades at all study intervals (p<0.01) except Dorifill and Apexit plus , there was no significant difference in growth-inhibition area grade among them. MTA Fillapex beard more growth-inhibition diameter in comparison to three other sealers (p<0.01). Conclusions: MTA Fillapex sealer has more antibacterial effect rather than Epiphany,Dorifill and Apexit plus on Enterococcus faecalis. The antibacterial effect of sealers decreases as time elapses.