Investigating the effect of Thymosin beta4 on the expression of HMGA1,HMGA2,HMGB1,HMGB2 on monocyte cells of mild multiple sclerosis type RRMS

Abstract

Multiple sclerosis (MS) is a long-term (chronic) disease of the central nervous system. It is thought to be an autoimmune disorder, a condition in which the body mistakenly attacks itself. MS is an unpredictable disease that affects people differently. Some people with MS may have only mild symptoms. When communication between the brain and other parts of the body is disrupted, others may lose the ability to see clearly, write, speak, or walk. Materials and methods: 30 MS patients referred to the MS clinic of Imam Reza Hospital with mild stages were selected, and blood samples were taken from the patients, and monocyte cells from the MACS brush were separated from PBMC. Bodi CD14. Monocyte cells were then cultured and stimulated with thymosin beta 4 proteins in a time course of two, eight and twenty-four hours. After stimulation, the cells were collected and the expression levels of HMGB1, HMGB2, HMGA1, HMGA2 proteins were evaluated by western blotting. Results: The expression level of HMGA1, HMGB2 did not change with the passage of time, but the expression level of HMGA2, HMGB1 increased in the final hours of stimulation. Conclusion: The results of western blot showed that thymosin beta 4 significantly increases the expression of HMGA2 and HMGB1. Keywords: thymosin beta 4- HMGB1-HMGB2-HMGA1-HMGA2 – multiple sclerosis Investigating the effect of Thymosin beta4 on the expression of HMGA1,HMGA2,HMGB1,HMGB2 on monocyte cells of mild multiple sclerosis type RRMS. Khodayi SH, Sandoghchian Shotorbani S Ph.D, Abolhasani S.M.D. Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences Introduction: Multiple sclerosis (MS) is a long-term (chronic) disease of the central nervous system. It is thought to be an autoimmune disorder, a condition in which the body mistakenly attacks itself. MS is an unpredictable disease that affects people differently. Some people with MS may have only mild symptoms. When communication between the brain and other parts of the body is disrupted, others may lose the ability to see clearly, write, speak, or walk. Materials and methods: 30 MS patients referred to the MS clinic of Imam Reza Hospital with mild stages were selected, and blood samples were taken from the patients, and monocyte cells from the MACS brush were separated from PBMC. Bodi CD14. Monocyte cells were then cultured and stimulated with thymosin beta 4 proteins in a time course of two, eight and twenty-four hours. After stimulation, the cells were collected and the expression levels of HMGB1, HMGB2, HMGA1, HMGA2 proteins were evaluated by western blotting. Results: The expression level of HMGA1, HMGB2 did not change with the passage of time, but the expression level of HMGA2, HMGB1 increased in the final hours of stimulation.