Evaluation of E-cadherin gene expression in monocytes isolated from stroke patients by stimulation of HMGB1, HMGB2

Abstract

Stroke remains a leading cause of morbidity and mortality worldwide, often associated with significant inflammation. High-mobility group proteins, particularly HMGA1 and HMGB2, play critical roles in inflammatory responses. This study aims to evaluate the expression levels of HMGA1 and HMGB2 in monocytes isolated from stroke patients and their implications in stroke pathology. Methods and Materials: Monocytes were isolated from stroke patients and stimulated to assess the expression levels of HMGA1 and HMGB2 at multiple time points (0h, 2h, 6h, 12h, 24h, and 48h). Quantitative PCR was employed to measure fold changes in gene expression over time. Comparative analyses were conducted to delineate the expression profiles of HMGA1 and HMGB2.

Results: HMGA1 expression exhibited a significant, time-dependent increase, peaking at 48 hours with nearly 100-fold change, while HMGB2 showed a more moderate increase, reaching around 60-fold by 48 hours. The data indicated a pronounced upregulation of HMGA1 compared to HMGB2, suggesting a differential role in the inflammatory response.