dc.contributor.advisor | Shanehbandi, Dariush | |
dc.contributor.author | Imani, Reza | |
dc.date.accessioned | 2025-07-15T09:23:47Z | |
dc.date.available | 2025-07-15T09:23:47Z | |
dc.date.issued | 2024 | en_US |
dc.identifier.uri | https://dspace.tbzmed.ac.ir:443/xmlui/handle/123456789/72548 | |
dc.description.abstract | Introduction
Recently, various studies have focused on the therapeutic potential of shikonin in various human malignancies, including osteosarcoma. However, the underlying mechanisms in shikonin-mediated anticancer effects are still not fully understood. Therefore, the present study investigated the effect of Linarin on Doxorubicin-induced apoptosis in SAOS-2 cells.
Methods
SAOS-2 cells were treated with Doxorubicin, Linarin and a combination of both, and cell viability was evaluated by MTT method. The mRNA expression of Bax, Bcl-2, and Caspase-9 was evaluated using qRT-PCR. Apoptosis was also investigated by flow cytometry.
Results
Doxorubicin resulted in significant inhibition of cell proliferation in a dose-dependent manner. The combination of doxorubicin and linarin resulted in significant inhibition of proliferation compared to single treatments (P<0.05). Linarin also induced apoptosis through modulating the expression of Bax, Bcl-2 and caspase-9. Furthermore, shikonin increased cisplatin-induced apoptosis in SAOS-2 cells. | en_US |
dc.language.iso | fa | en_US |
dc.publisher | Tabriz University of Medical Sciences, Faculty of Medicine | en_US |
dc.relation.isversionof | https://dspace.tbzmed.ac.ir:443/xmlui/handle/123456789/72547 | en_US |
dc.subject | osteosarcoma | en_US |
dc.subject | doxorubicin | en_US |
dc.subject | apoptosis | en_US |
dc.subject | linarin | en_US |
dc.title | Investigating the effect of linarin in increasing the sensitivity of osteosarcoma cells to doxorubicin | en_US |
dc.type | Thesis | en_US |
dc.contributor.supervisor | Yousefi, Bahman | |
dc.identifier.docno | 6012009 | en_US |
dc.identifier.callno | 12009 | en_US |
dc.description.discipline | Medicine | en_US |
dc.description.degree | MD Degree | en_US |