Evaluation of SKI gene expression by activation of TLR2 and TLR4 in monocyte cells of stroke patients

Abstract

Stroke is one of the most important disabling diseases in the world and one of the most important causes of death. In this study, the level of the SKI gene was studied, which plays a fundamental role in the level of dementia caused by stroke. For this reason, this gene is studied by stimulating the receptors of the innate immune system in order to find a way to inhibit this gene. Methods and Materials: In this case-control study, 5 cc of blood was collected from 20 stroke patients referred to the neurology department of Imam Reza Hospital and 10 normal individuals. Blood monocyte cells were isolated using the PBMC technique and CD14 antibody was used in flow cytometry technique for purity percentage. The cells were cultured and stimulated with TLR2 and TLR4 from Invivogen Company in a time course of 6, 12, 24 and 48 hours. Then, the cells were collected after the end of the culture and cell mRNA was extracted and the expression level of the SKI gene was evaluated through the REAL TIME PCR technique. Data analysis was performed with SPSS17 software. Results: The results showed that both TLR2 and TLR 4stimulation resulted in a significant increase in SKI gene expression in monocytes of stroke patients (p<0.05). This increase peaked at 12 hours after movement. There was no significant difference between TLR2 and TLR4, and both increased to the same extent.