Evaluation of Endometrial receptivity-related genes and endometrial histological changes in PCOS mice

Abstract

PCOS is one of the common metabolic and endocrine diseases in women of reproductive age. Genetic, hormonal, and metabolic factors can be considered effective in causing this syndrome. PCO has different negative effects on people's fertility, so that people with this syndrome face a decrease in fertility and even an increase in spontaneous abortion. One of the important reasons for the decrease in fertility in these people is the decrease in endometrial receptivity. This word means the successful attachment of the blastocyst to the uterine endometrium. Due to the high prevalence of PCOS and unclear etiology and effects, this article was conducted with the aim of investigating endometrial receptivity in PCOS patients.

Method: In this method, an animal model of polycystic ovary is induced using estradiol valerate at a dose of 40 mg for 60 days, and adult female rats are divided into six groups after confirming the induction of polycystic ovary using vaginal smear. Rats that do not have a regular cycle will be excluded from the study. The mice with polycystic ovary model will be treated with hormones during the estrous phase and then anesthetized with ketamine and xylazine. After extraction, uterine tissue was used for histological and histochemical studies and gene expression analysis. Finding: Our results show that the thickness in the PCOS group was lower than in other treatment groups, and an increase in thickness in this group could reduce endometrial receptivity. On the other hand, using hormone therapy, a significant reduction in thickness was observed compared to the PCOS group. In the study of PR gene, the highest level was related to the control group and other treatment groups had a significant decrease compared to the control group. In the PCOS group, there was an increase in AR gene expression compared to the control group and the estrogen, FSH and HCG treatment group showed a significant decrease compared to the PCOS group. In the study of ER-a gene expression, it was also found that there was a significant decrease in the FSH and LH+FSH treatment group compared to the control group. HOXA10 and LIF are involved in endometrial receptivity and are impaired in the PCOS group. In immunohistochemical analysis, the expression intensity of both HOXA and ER proteins was high in the control group, and the other treatment groups had a significant decrease.