Study the impact of co-inhibition of IL-6 and β-catenin in cancer cells

Abstract

Cancer treatment is one of the main challenges for researchers in this field. Common methods of cancer treatment, including chemotherapy, radiotherapy, and surgery, are not effective in many patients, and many patients face drug resistance, disease recurrence, and severe treatment complications. Therefore, it seems very necessary to find new and effective treatment methods for cancer treatment. There are many factors in the tumor microenvironment that contribute to tumor growth and spread and suppress antitumor immune responses. IL-6 and β-catenin are two very important factors in the tumor microenvironment that stimulate each other in an additive loop and contribute to tumor growth. Therefore, in this study, these two factors were targeted in combination.

Materials and methods: In this study, chitosan-based nanoparticles loaded with siRNA molecules were used to target IL-6 and β-catenin factors. This study was conducted on two cell lines, 4T1 and CT26, which are mouse cancer cell lines. The effect of treatment on the survival of cells was investigated by the MTT method and the effect of treatment on the expression of target genes was done by the Real-time PCR assay. Results: The results of this study showed that simultaneous targeting of IL-6 and β-catenin factors significantly reduced the survival of cancer cells. Also, suppressing the expression of these two factors changed the expression pattern of genes involved in apoptosis, metastasis, and proliferation. and cellular angiogenesis.