Evaluation of gene expression NEAT1, HNF1A-AS1 and MVIH in patients with colorectal cancer and normal healthy people as controls

Abstract

Colorectal cancer is one of the most common cancers in the world, and its prevalence has increased significantly in recent decades due to changes in the life cycle of people. Genetics and the environment have a separate and clear role in this cancer. Although the role of the environment in this disease is more prominent than genetics, but these environmental changes eventually lead to cell death by causing genetic changes. LNC-RNAs There is a group of non-coding genes that, due to their biological role and changes, can be used as biomarkers in the diagnosis of various diseases such as cancer. mNEAT1, HNF1A-AS1 and MVIH are among the most well-known non-coding RNAs whose expression changes in cancer have been proven and their role has been evaluated as a diagnostic factor. The aim of this study was to investigate the relative changes in the expression levels of these genes in serum samples of patients with colon cancer. Methods: During this study, serum samples from 50 patients and 50 samples from healthy individuals were collected as a control sample. After collecting RNA related to the samples, the samples were extracted and the expression of the desired gene was evaluated using real-time PCR. Differences in expression levels of the two groups were assessed by appropriate statistical tests. In statistical tests, Pvalue <0.05 was considered significant. Results: The expression of all genes studied in this study had significant changes in the patient group compared to the control group. But these changes were not related to any of the clinicopathological features of the patients.