| dc.description.abstract | Abstract
Background: One of the most important materials needed for root canal therapy is sealer. The aim of this study was to assess tissue reaction of a new polycaprolacton based root canal sealer in rat model.
Materials and methods:
Eighty adult male rats were randomly divided into 5 groups (n =16 for assessment at 3, 7, 15, 30, and 60 day intervals). The rats were anesthetized by Ketamine and Xylazine. Surgical anesthesia lasted thirty minutes. After shaving their back hair, and cleaning the spot with iodine 5%, two grooves with a length of 1 cm were created with scalpel no. Thus, one of the tested materials was randomly inserted into each of the incisions and an empty polyethylene angioket tube with a length of 8 mm and an internal diameter of 1.1 mm was inserted into one of the incision site as a control. After the 3, 7, 15, 30, and 60 day intervals, the rats were anesthetized again. The rats were sacrificed by overdosing anesthetic drug injection (barbiturate or ether). The samples were kept in 10% formalin and then sent to prepare slides. After preparing the paraffin blocks, longitudinal sections were prepared along the axis of the tubes with a thickness of 6 μ passing through the studied material. Four incisions were prepared from each block, passing longitudinally through the largest diameter of the tube.
Results: The results showed that there was no significant difference between the case and control groups in the degree of inflammation and the cell count (lymphocyte, macrophages, mast cell, plasma cell, neutrophil, eosinophil) in the study groups for two month evaluations (P-value < 0.05).
Conclusion: The studied new sealer was tissue biocompatible in animal rat model.
Keywords: Root canal sealer, Polycaprolacton, Biocompatibility, Rat model, Endodontics | en_US |
