The hemostatic effects of a novel gelatin sponge containing aloe vera nanoparticles in rat animal model

Abstract

Introduction: Biodegradable hemostat sponges are new materials in dentistry that are used to quickly stop blood after tooth extraction. The purpose of this study was to investigate the hemostatic effects of a new gelatin sponge containing aloe vera nanoparticles in rat animal model. Methods: For this purpose, nine adult Wistar rats were purchased for this research. The animals were divided into three groups of test, negative control, and positive control. Rats were anesthetized with ketamine and xylazine. A small incision was made on the femoral artery and 100 mg of the tested hemostat was pressed on it and the coagulation time was recorded with a stopwatch. Gelatin sponge without aloe vera nanoparticles was considered as a positive control group. In the negative control group, no substance was used and the normal coagulation time was recorded. The comparison of the mean values between the studied groups was done with the Kruskal-Wallis test. SPSS version 25 software was used for data analysis with a significant level of 0.05. Results: The new hemostat sponge had a faster blood clotting time than both positive and negative control groups (P=0.04). Blood clotting time was 17.6±1.0 s for the test group, 210±0.86 s for the negative control group, and 20±1.2 s for the positive control. Conclusion: The new gelatin sponge containing aloe vera nanoparticles can be used to control bleeding during surgery or tooth extraction.