Effect of carvacrol on the rwgrneration of lung tissue in tyoe1 diabetic mice via Nrf2/TLR4 axis

Abstract

So far, the effectiveness of carvacrol in animal models of diabetes and lung diseases has been proven in various studies. However, the main mechanism of the therapeutic effects of this drug has not been determined. Therefore, in this study, the effect of carvacrol on the process of lung tissue repair in mice with type 1 diabetes was investigated through the Nrf2/TLR4 axis. Methods In this study, 24 adult male mice were randomly divided into 3 groups (n=8 for each group) including control group, diabetes group, and diabetes + carvacrol group. In order to induce diabetes in diabetic groups, intraperitoneal injection of streptozotocin (50 mg/kg) was used. In the group receiving carvacrol, two weeks after the induction of diabetes, carvacrol was injected intraperitoneally (50 mg/kbw) every other day for four weeks. 48 hours after the last injection, the animals were killed by injecting a high dose of ketamine and xylazine, and their lung tissue was removed for pathological examination and expression of the desired genes. Results The findings of this study showed increased pathological changes, increased expression of TLR4, MYD88, TRIF and IRAK-1 and decreased expression of Nrf2 in the lung tissue of diabetic groups compared to the control group. Carvacrol injection improved the levels of these parameters.