Study of simultaneous silencing of STAT3 and CD155 in 4T1 and CT26 cancer cell lines

Abstract

Although chemotherapy, radiotherapy and surgery are still the first choice for the treatment of almost all cancers, their effectiveness is doubtful in many cases because it has been associated with resistance to treatment, recurrence and side effects. Therefore, new treatment methods such as immunotherapy have emerged to treat cancer. Therefore, targeting the effective factors in cancer growth can be an effective method in cancer treatment. STAT3 and CD155 factors in the tumor microenvironment strengthen each other in an additive loop and cause tumor growth. Therefore, in this study, these two factors were targeted for treatment in cancer cells. Methods: This study was conducted on two mouse cancer cell lines including 4T1 and CT26. Nanoparticles loaded with siRNA molecules were used to target STAT3 and CD155 factors. The effect of treatment on the survival of cells was evaluated by MTT method and the effect of treatment on the expression of target genes was evaluated by Real-time PCR method. Results: The results of this study showed that simultaneous suppression of the expression of STAT3 and CD155 factors significantly reduces the survival of cancer cells. Also, combined treatment changed the expression of genes involved in cell survival, angiogenesis, and metastasis.