The effect of miR-301 on BMI gene expression in A-375 melanoma cell line

Abstract

Introduction: BMIn cancer is one of the most common cancers in the world. One out of every three cancers diagnosed in the world is BMIn cancer, and the importance of early treatment is increasing day by day. Melanoma is the most aggressive and deadly type of BMIn cancer and one of the most malignant cancers. Various methods such as radiotherapy, chemotherapy, immunotherapy and surgery are used in the treatment of this type of cancer. Materials and methods: The present laboratory study was performed on melanoma cancer cells in RPMI640 culture medium containing 10% FCS at 37ₒc and 5% CO2. After counting and dividing the cells, the inside of the cell plate was stimulated with MiR301. Incubation was done. After 48 hours, the cells were collected and total mRNA was extracted and the expression level of BMI was checked through qRT-PCR. Statistical tests P < 0.05 were considered significant. Results: The results of the present study showed that the expression level of TLR2 and TLR4 IL6 in melanoma cell line (A-375) decreased when exposed to HSP70. Also, the results showed the level of MAPK expression in melanoma cell line