Investigating the effects of Raptinal in increasing the sensitivity of MG-63 cells to Paclitaxel

Abstract

Recently, various studies have focused on the therapeutic potential of Raptinal in various human malignancies, including osteosarcoma. However, the underlying mechanisms in Raptinal-mediated anticancer effects are still not fully understood. Therefore, the present study investigated the effect of Raptinal on paclitaxel-induced apoptosis in MG-63 cells. Methods MG-63 cells were treated with paclitaxel, Raptinal and a combination of both, and cell viability was evaluated by MTT method. The mRNA expression of Bax, Bcl-2 and Caspase-3 evaluated using qRT-PCR. Apoptosis was also investigated by flow cytometry. Results Paclitaxel resulted in significant inhibition of cell proliferation in a dose-dependent manner. The combination of paclitaxel and Raptinal resulted in significant inhibition of proliferation compared to single treatments (P<0.05). Raptinal also induced apoptosis through modulating the expression of Bax, Bcl-2 and Caspase-3. Furthermore, Raptinal increased paclitaxel-induced apoptosis in MG-63 cells.