Design and development of metal-organic framework-based nanocomposite hydrogels for quantification of deferiprone in exhaled breath condensate

Abstract

Introduction: In the present study, a spectrofluorimetric method based on nanocomposite hydrogel containing metal-organic framework (MOF) was presented to determine the concentration of deferiprone in exhaled breathe condensate samples. In the preparation of the desired nanocomposite, the MOF was inserted into the hydrogel matrix by post-synthetic method and was used as a nanosensor to determine the amount of deferiprone.Objectives: The purpose of this project is to use a sensor based on nanocomposite hydrogel containing a MOF for the quantification of deferiprone in exhaled breathe condensate samples.Methodology: In the first step, MOF nanoparticles were synthesized. In this way, the MIL-101 MOFs nanoparticle will be synthesized by hydrothermal method and then will be inserted into the agarose hydrogel and will be used as a sensor to determine the amount of deferiprone drug. For this purpose, the blank samples which include MIL-101/agarose nanocomposite hydrogel and buffer with suitable pH are placed in the spectrofluorimeter cuvette and its emission spectrum is drawn. Then, the emission spectrum of the samples containing defriprone along with the reagents used in the blank sample is drawn too and the difference in the peak intensity of the two graphs will be recorded as the analytical response. After validation of the proposed method, drug tracking will be done in exhaled breathe condensate samples.Results: Various parameters affecting the performance of the nanosensor were investigated and optimized. Under optimal conditions, the designed nanosensor showed a suitable analytical performance for determining the amount of deferiprone in a wide linear range from 0.005 to 1.5 μg mL-1 with a limit of detection 0.003 μg mL-1.Discussion and conclusion: The mentioned nanosensor has the advantages of high sensitivity, reproducibility and selectivity compared to deferiprone drug. These characteristics show that the proposed nanosensor can be a reliable tool in the identification of deferiprone.