Investigation of the effect of the nanoparticles loaded with siRNA molecules against HIF-1α along with BV6 on the 4T1 tumor cell’s proliferation and viability

Abstract

A wide network of different factors in the tumor microenvironment in cooperation with each other cause tumor growth and suppression of immune responses. Individual inhibition of these factors is usually simply masked by the compensatory action of another factor and tumor growth continues. Therefore, the combined targeting of factors involved in tumor growth can be considered a suitable solution for cancer treatment. Hypoxia created in solid tumors aggravates many pathways related to cancer growth, including the pathway of cancer cell survival. Therefore, in this study, it was decided to inhibit the growth of cancer cells by inhibiting HIF-1a factor in combination with BV6 drug (apoptosis inducer). Materials and methods: 4T1 mouse breast cancer cell line was used in this study. In order to investigate the effect of treatment on cell survival, MTT method was used. Real-time PCR method was also used to evaluate the expression of target genes. Result: The results of this study showed that combined treatment of cancer cells reduces the survival of cancer cells. Also, combined treatment changed the expression of genes involved in the growth of cancer cells.