Effect of Carvacrol on histological damage and Bax and BCl2 gene expression on ovary and oxidative stress and hormonal level in blood of adult rats after ovarian torsion/detorsion

Abstract

Torsion followed by ovarian detorsion or in other words ovarian ischemia/reperfusion is a pathophysiological event. Because the histological damage caused by torsion of the ovary is observed with a decrease in blood flow followed by a lack of oxygen (ischemia) in the ovarian tissue. Therefore, the present study was conducted with the aim of investigating the protective effect of carvacrol on bax and bcl-2 gene expression and histological damage and oxidative stress caused by ovarian torsion in adult rats.

Materian and Method: In this study The rats were randomly divided into 4 groups. Sham (control) group, a torsion/detorsion group and a torsion/detortion treatment group with a dose of 75mg/kg carvacrol and a healthy group receiving carvacrol with a dose of 75mg/kg. After 10 days of treatment, the ovaries were removed for tissue examination and blood sampling was done to evaluate the serum level of antioxidant enzymes and to examine the expression of bax and bcl-2 genes.

Results: Histological damage caused by torsion/detorsion in groups receiving carvacrol Is decreased compared to the torsion group. Estrogen, FSH, and LH hormones increased in the treatment groups compared to the torsion group While the amount of testosterone decreased.Also, the expression of bax gene decreased in the groups receiving carvacrol compared to the control and torsion groups. Conversely, bcl-2 gene expression increased in treatment groups. The level of MDA in the treatment groups showed a significant decrease and the level of SOD and GPX increased.