Evaluation of TG (rs2069556) and IL-1β (rs16944) gene polymorphisms in individuals with anti-TPO-positive serum levels and healthy individuals with normal Anti-TPO

Abstract

Many studies have shown that inflammatory cytokines such as interleukin 1 beta (IL-1) contribute to the development of autoimmune thyroid diseases (AITDs). The present study was conducted with the aim of investigating polymorphisms of thyroglobulin gene (rs2069556) and IL-1β gene (rs16944) in Anti-TPO positive patients. Methods: In this clinical trial study, 82 Anti-TPO positive patients (average age 39.95 ± 8.16 years) and 82 healthy individuals with an average age of 40.74 ± 1.23 years were introduced by the laboratory. Then the serum level of anti-TPO vitamin was measured. After blood sampling and DNA extraction, polymorphisms of thyroglobulin (rs2069556) and IL-1β gene (rs16944) were investigated using the tetra-ARMs PCR technique. Results: AG rs16944 genotype reduces the risk of disease and increases a person's health, but in the case of AA genotype, it increases the risk of disease. Also, compared to allele A, G allele reduces the risk of disease and increases a person's health. Examination of Hardy-Weinberg equilibrium at the locus Rs16944 showed that the difference observed for the observed and expected genotypes between the two groups was significant and the population was out of equilibrium. On the other hand, the frequency of Rs2096556 genotypes between the studied groups is not significant. But the G allele increases the risk of disease and reduces the health of a person compared to the A allele. Also, the difference observed for the observed and expected genotypes between the two groups is significant and the population is not in balance.