To study of the presence of surface proteins LPxTG and genomic islans arginine catabolic mobile element and their effect on antibiotic resistance and biofilm production in staphylococcus epidermidis clinical isolated from patients and healthy employees of sina and childrens hospitals, Tabriz, during the year 1399

Abstract

Staphylococcus epidermidis is the most abundant natural microbiota of the skin and mucous membranes. This bacterium is the main cause of infections related to medical instruments, especially catheters with high biofilm production power. The purpose of this case-control study was to investigate the presence of virulence factors and its effect on antibiotic resistance and biofilm production in Staphylococcus epidermidis and to compare them between clinical strains and normal skin flora of people working in Sinai and children's hospitals, Tabriz. Materials and methods: Among the 103 samples collected from patients hospitalized in different departments of Sinai and Tabriz Children's Hospitals, a total of 48 clinical Staphylococcus epidermidis isolates were collected. also from 118 skin samples we collected from the medical staff of these hospitals 48 Staphylococcus epidermidis normal flora isolates were obtained. Routine phenotypic tests and and SesC and tuf PCR tests were performed in the microbiology laboratory of the faculty Tabriz Medicine for molecular diagnosis. Then the pattern of antibiotic sensitivity was determined by disk diffusion method. Next, PCR test was performed to check the presence of icaABCD, altE, sdrG, sesK, sesM, sesB, sesL, arc and opp3 genes. Finally, biofilm production was investigated by microtitreplate method and statistical analysis by SPSS software. Results: Of the total isolates, 79 (82.3%) were sesC positive and 90 (93.8%) were tuf positive. In resistance to antibiotics, the highest resistance to penicillin was 100%, erythromycin 91.7%, cefoxetine , clindamycin was 59.4%, saprofloxacin 38.5%, cotrimoxazole , gentamicin 32.3%, amikacin 24%, rifampin 2.1%, linezolid  and vancomycin 0%. Also, the frequency of methicillin resistance gene mecA was equal to 75%. The frequency of sesMBLK genes was .5%, 59.4%, 0% and 3.1%, respectively. The frequency of weak biofilm was 13.5%, medium 58.3% and strong biofilm was 28.1%. The frequency of atlE and sdrG genes was 84.4% and 13.5%, respectively; The frequency of icaABCD genes among isolates was 51%, 24%, 26% and 25.6%, respectively. The frequency of arc and opp3 genes was % and 12.5%.